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Shanmugapriya, M.
- Production and Characterization of Partially Purified Chitosanase Enzyme of Bacillus cereus A4/B4 Isolated from Biowaste Soil Samples:Bioactive Chitooligosaccharide
Abstract Views :528 |
PDF Views:416
Authors
P. Vanathi
1,
S. Fernandez
1,
M. Shanmugapriya
1,
T. S. Riyamol
1,
V. Loganathan
1,
V. I. Hairul-Islam
2,
P. Premasudha
3,
R. Rajendran
4
Affiliations
1 Department of Microbiology, Maharaja Co Education Arts & Science College, Perundurai, IN
2 Division of Microbiology and immunology, Pondicherry centre for biological sciences, Pondicherry, IN
3 Department of Nanotechnology, Bharathiar University, Coimbatore, IN
4 PSG College of Arts & Science, Coimbatore, IN
1 Department of Microbiology, Maharaja Co Education Arts & Science College, Perundurai, IN
2 Division of Microbiology and immunology, Pondicherry centre for biological sciences, Pondicherry, IN
3 Department of Nanotechnology, Bharathiar University, Coimbatore, IN
4 PSG College of Arts & Science, Coimbatore, IN
Source
FoodSci: Indian Journal of Research in Food Science and Nutrition, Vol 2, No 2 (2015), Pagination: 41-48Abstract
Microbial chitosanase has received the attention of producing chitooligosaccharides. In this study, Chitooligosaccharide was developed by chitosanase enzyme hydrolysis and evaluated for its bioactivity. Chitosan degrading Bacillus cereus was isolated from biowaste sample and its extracellular enzyme chitosanase was characterized. The chitosanase enzyme production was induced by chitosan substrate and the enzyme production reached the maximum in 72 hours. The optimum pH and temperature for the production was 6.5 and 30°C respectively. The culture medium with 1.0 to 1.5% of substrate level with xylose served as significantly for enzyme production. The molecular weight of the enzyme 43KDa was determined by SDS-PAGE. The chitooligosaccharide produced by chitosanase hydrolysis inhibited the growth of the pathogens and showed MIC50 as 0.2 μg/ml against all the test pathogens and has proved to be a potential antimicrobial agent against skin and other infections.Keywords
Bacillus Species, Bioproduction, Chitosanase, Chitooligosaccharides, Microbial Enzymes.References
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- Behaviour of Cold Formed Columns under Axial Loading with Quartz and Silica In-Filled Concrete
Abstract Views :204 |
PDF Views:0
Authors
Affiliations
1 Department of Civil Engineering, Sathyabama University, Chennai - 600119, Tamil Nadu, IN
2 Department of Civil Engineering, VIT University, Chennai - 600127, Tamil Nadu, IN
1 Department of Civil Engineering, Sathyabama University, Chennai - 600119, Tamil Nadu, IN
2 Department of Civil Engineering, VIT University, Chennai - 600127, Tamil Nadu, IN
Source
Indian Journal of Science and Technology, Vol 9, No 5 (2016), Pagination:Abstract
The experimental investigation was carried to study the investigate the behaviour of hollow, Plain Cement Concrete (PCC) and Silica; Quartz replaced concrete in-filled medium light gauge columns subjected to axial loads. The Cold formed sheets were welded continuously welded at the middle along its length. The basic study for the infill materials were carried out initially. For PCC in-fill M40 grade concrete were chosen. A total of 04 columns of 1.50m length were tested, without in fill, with plain concrete in-fill and with Quartz and Silica in-fill having a cross section of 100 x 50x 2mm. All the specimens were tested to failure. Ultimate load, Load – Axial shortening and Load-Strain for all the specimens tested were plotted. With the comparison of hollow columns the Quartz concrete in-filled columns take around 50% more ultimate loads when loaded axially. The in-filled column with Quartz withstands the maximum load; this is due to the fineness modulus property of the material. The Euro Code 4 predicted the ultimate loads closer to that of the experimental loads. Thus Quartz Sand can be used as an alternate filler material.Keywords
Aggregate, Cold Formed, Concrete, In-Filled, Quartz, Silica- Parametric Analysis of a Miniaturized Inverted ? Shaped Antenna for Wireless Sensor Network Applications
Abstract Views :184 |
PDF Views:0
Authors
Affiliations
1 Department of Electronics and Communication Engineering, M.A.M. College of Engineering, IN
2 M.A.M. College of Engineering, IN
1 Department of Electronics and Communication Engineering, M.A.M. College of Engineering, IN
2 M.A.M. College of Engineering, IN